Detection and Quantitative Determination of Lipid Peroxidation in Living Bodies. Thiobarbituric Acid Test.
نویسندگان
چکیده
منابع مشابه
Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation.
The thiobarbituric acid (TBA) reaction, quantified by colorimetry or fluorimetry, is the method most widely used for studying lipid peroxidation in both laboratory animals and in humans with disorders. However, concerns regarding its analytical specificity have often been expressed, because TBA reacts with a wide variety of chemical species to produce a pink to red color. In this study, we reac...
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Electrochemical oxidation of iodide has been studied in the presence of 2-thioborbituric acid using cyclic voltammetry and controlled-potential coulometry. The results indicate that, the resulting iodine takes part in halogenations reaction with 2-thiobarbituric acid. In addition, the present data are indicative of the suitability of iodide as a quasi mediator for determination of 2-thiobar...
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Lipid peroxides in tissues were fluorometrically determined using 1,3-diphenyl-2-thiobarbituric acid (DPTBA) as a fluorogenic reagent, and the results were compared with those obtained by the DPTBA-visible method, thiobarbituric acid (TBA)-fluorescence method, or TBA-visible method. The optimum conditions for determining lipid peroxides were analyzed by using brain homogenate. The optimum pH fo...
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The aim of this work was to compare the methods of malondialdehyde detection, as the main secondary product of the lipid peroxidation process, in meat and meat products. Malondialdehyde measurements were performed by two modified methods, the 2-thiobarbituric acid spectrophotometric method and the reverse-phase high-performance liquid chromatography in raw, mechanically-deboned chicken meat and...
متن کاملDetection and quantitative assay of 2 - thiobarbituric acid from inert solid and solubilized samples
The thiobarbiturates differ from other barbiturates in having a sulfur atom to replace the carbonyl oxygen at the C-2 position. The drug 2-thiobarbituric acid was soluble in 95% ethanol and 5% water for sensitive detection and measurement. All samples were examined at 340 nanometer wavelength. An ultraviolet-visible instrument with one centimeter glass cuvettes was suitable for this assay. The ...
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ژورنال
عنوان ژورنال: Eisei kagaku
سال: 1993
ISSN: 0013-273X
DOI: 10.1248/jhs1956.39.1